Composite
Part:BBa_K571006:Design
Designed by: Lu-Chu Ke Group: iGEM11_TzuChiU_Formosa (2011-10-03)
R0011+acsCD/pSB1C3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3233
Illegal PstI site found at 143
Illegal PstI site found at 329
Illegal PstI site found at 779
Illegal PstI site found at 2615
Illegal PstI site found at 2945
Illegal PstI site found at 3071
Illegal PstI site found at 4144 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3233
Illegal PstI site found at 143
Illegal PstI site found at 329
Illegal PstI site found at 779
Illegal PstI site found at 2615
Illegal PstI site found at 2945
Illegal PstI site found at 3071
Illegal PstI site found at 4144 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3233
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3233
Illegal PstI site found at 143
Illegal PstI site found at 329
Illegal PstI site found at 779
Illegal PstI site found at 2615
Illegal PstI site found at 2945
Illegal PstI site found at 3071
Illegal PstI site found at 4144 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3233
Illegal PstI site found at 143
Illegal PstI site found at 329
Illegal PstI site found at 779
Illegal PstI site found at 2615
Illegal PstI site found at 2945
Illegal PstI site found at 3071
Illegal PstI site found at 4144
Illegal NgoMIV site found at 662
Illegal NgoMIV site found at 1738
Illegal NgoMIV site found at 2858
Illegal AgeI site found at 874
Illegal AgeI site found at 2165 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is a very long sequence, therefore below is a few measures to take note during cloning.
- The concentration agarose gel during gel electrophoresis should be about 0.8-1%
- The enzyme digestion reaction time is advised to be elongated so that digestion could be more perfect.
- The respective restriction enzyme at the both end of primer should not cut the sequence of another gene.
Source
- acsCD: GenBank AB091060.1
- promoter: iGEM registry of standard biological part